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Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2

Reverse transcription (RT)-PCR, the principal diagnostic method applied in the world-wide struggle against COVID-19, is capable of detecting a single molecule of a viral genome. Correctly designed and practiced RT-PCR assays for SARS-CoV-2 should not cross react with similar but distinct viral patho...

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Autores principales: Huggett, Jim F, Benes, Vladimir, Bustin, Stephen A, Garson, Jeremy A, Harris, Karthyn, Kammel, Martin, Kubista, Mikael, McHugh, Timothy D, Moran-Gilad, Jacob, Nolan, Tania, Pfaffl, Michael W, Salit, Marc, Shipley, Greg, Vallone, Peter M, Vandesompele, Jo, Wittwer, Carl, Zeichhardt, Heinz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7499524/
https://www.ncbi.nlm.nih.gov/pubmed/32894756
http://dx.doi.org/10.1093/clinchem/hvaa214
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author Huggett, Jim F
Benes, Vladimir
Bustin, Stephen A
Garson, Jeremy A
Harris, Karthyn
Kammel, Martin
Kubista, Mikael
McHugh, Timothy D
Moran-Gilad, Jacob
Nolan, Tania
Pfaffl, Michael W
Salit, Marc
Shipley, Greg
Vallone, Peter M
Vandesompele, Jo
Wittwer, Carl
Zeichhardt, Heinz
author_facet Huggett, Jim F
Benes, Vladimir
Bustin, Stephen A
Garson, Jeremy A
Harris, Karthyn
Kammel, Martin
Kubista, Mikael
McHugh, Timothy D
Moran-Gilad, Jacob
Nolan, Tania
Pfaffl, Michael W
Salit, Marc
Shipley, Greg
Vallone, Peter M
Vandesompele, Jo
Wittwer, Carl
Zeichhardt, Heinz
author_sort Huggett, Jim F
collection PubMed
description Reverse transcription (RT)-PCR, the principal diagnostic method applied in the world-wide struggle against COVID-19, is capable of detecting a single molecule of a viral genome. Correctly designed and practiced RT-PCR assays for SARS-CoV-2 should not cross react with similar but distinct viral pathogens, such as the coronaviruses associated with the common cold, and should perform with very high analytical sensitivity. This analytical performance is predicated on the ability of the method to detect the presence of the selected nucleic acid target, without detection of a false positive signal.
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spelling pubmed-74995242020-09-21 Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2 Huggett, Jim F Benes, Vladimir Bustin, Stephen A Garson, Jeremy A Harris, Karthyn Kammel, Martin Kubista, Mikael McHugh, Timothy D Moran-Gilad, Jacob Nolan, Tania Pfaffl, Michael W Salit, Marc Shipley, Greg Vallone, Peter M Vandesompele, Jo Wittwer, Carl Zeichhardt, Heinz Clin Chem Opinion Reverse transcription (RT)-PCR, the principal diagnostic method applied in the world-wide struggle against COVID-19, is capable of detecting a single molecule of a viral genome. Correctly designed and practiced RT-PCR assays for SARS-CoV-2 should not cross react with similar but distinct viral pathogens, such as the coronaviruses associated with the common cold, and should perform with very high analytical sensitivity. This analytical performance is predicated on the ability of the method to detect the presence of the selected nucleic acid target, without detection of a false positive signal. Oxford University Press 2020-09-07 /pmc/articles/PMC7499524/ /pubmed/32894756 http://dx.doi.org/10.1093/clinchem/hvaa214 Text en © American Association for Clinical Chemistry 2020. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Opinion
Huggett, Jim F
Benes, Vladimir
Bustin, Stephen A
Garson, Jeremy A
Harris, Karthyn
Kammel, Martin
Kubista, Mikael
McHugh, Timothy D
Moran-Gilad, Jacob
Nolan, Tania
Pfaffl, Michael W
Salit, Marc
Shipley, Greg
Vallone, Peter M
Vandesompele, Jo
Wittwer, Carl
Zeichhardt, Heinz
Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2
title Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2
title_full Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2
title_fullStr Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2
title_full_unstemmed Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2
title_short Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2
title_sort cautionary note on contamination of reagents used for molecular detection of sars-cov-2
topic Opinion
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7499524/
https://www.ncbi.nlm.nih.gov/pubmed/32894756
http://dx.doi.org/10.1093/clinchem/hvaa214
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