A Fluorescence‐Based Assay System for the Determination of Haloperoxidase‐Activity Using a Two‐Dimensional Calibration Ap‐proach

Screening for an interesting biocatalyst and its subsequent kinetic characterization depends on a reliable activity assay. In this work, a fluorometric assay based on the halogenation of 4‐methyl‐7‐diethylamino‐coumarin was established to monitor haloperoxidase‐activity. Since haloperoxidases utiliz...

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Detalles Bibliográficos
Autores principales: Fejzagić, Alexander V., Myllek, Sebastian, Hogenkamp, Fabian, Greb, Julian, Pietruszka, Jörg, Classen, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Full Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7499805/
https://www.ncbi.nlm.nih.gov/pubmed/32995110
http://dx.doi.org/10.1002/open.202000184

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7499805/
https://www.ncbi.nlm.nih.gov/pubmed/32995110
http://dx.doi.org/10.1002/open.202000184

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