Cargando…

Interdependency of regulatory effects of iron and riboflavin in the foodborne pathogen Shigella flexneri determined by integral transcriptomics

Shigella flexneri is the causative agent of dysentery. For pathogens, iron is a critical micronutrient as its bioavailability is usually low in bacterial niches. This metal is involved in critical physiological processes mainly as a component of important metabolic molecules involved in redox reacti...

Descripción completa

Detalles Bibliográficos
Autores principales: Lozano Aguirre, Luis Fernando, Salazar, Juan Carlos, Vásquez, José Ignacio, García-Angulo, Víctor Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7500357/
https://www.ncbi.nlm.nih.gov/pubmed/32983629
http://dx.doi.org/10.7717/peerj.9553
_version_ 1783583848628486144
author Lozano Aguirre, Luis Fernando
Salazar, Juan Carlos
Vásquez, José Ignacio
García-Angulo, Víctor Antonio
author_facet Lozano Aguirre, Luis Fernando
Salazar, Juan Carlos
Vásquez, José Ignacio
García-Angulo, Víctor Antonio
author_sort Lozano Aguirre, Luis Fernando
collection PubMed
description Shigella flexneri is the causative agent of dysentery. For pathogens, iron is a critical micronutrient as its bioavailability is usually low in bacterial niches. This metal is involved in critical physiological processes mainly as a component of important metabolic molecules involved in redox reactions. Usually bacteria respond to fluctuations in iron availability to regulate iron acquisition and other iron-related functions. Recently the close metabolic feedback between iron and riboflavin, another pivotal biological redox agent, began to draw attention in bacteria. This is a widespread biological phenomenon, partly characterized by the coordination of regulatory responses to iron and riboflavin, probably owed to the involvement of these cofactors in common processes. Nonetheless, no systematic analyses to determine the extent of this regulatory effect have been performed in any species. Here, the transcriptomics responses to iron, riboflavin, iron in the presence of riboflavin and riboflavin in the presence of iron were assessed and compared in S. flexneri. The riboflavin regulon had a 43% overlap with the iron regulon. Notably, the presence of riboflavin highly increased the number of iron-responsive genes. Reciprocally, iron drastically changed the pool of riboflavin-responsive genes. Gene ontology (GO) functional terms enrichment analysis showed that biological processes were distinctively enriched for each subgroup of responsive genes. Among the biological processes regulated by iron and riboflavin were iron uptake, amino acids metabolism and electron transfer for ATP synthesis. Thus, iron and riboflavin highly affect the transcriptomics responses induced by each other in S. flexneri. GO terms analysis suggests that iron and riboflavin coordinately regulate specific physiological functions involving redox metabolism.
format Online
Article
Text
id pubmed-7500357
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher PeerJ Inc.
record_format MEDLINE/PubMed
spelling pubmed-75003572020-09-25 Interdependency of regulatory effects of iron and riboflavin in the foodborne pathogen Shigella flexneri determined by integral transcriptomics Lozano Aguirre, Luis Fernando Salazar, Juan Carlos Vásquez, José Ignacio García-Angulo, Víctor Antonio PeerJ Genomics Shigella flexneri is the causative agent of dysentery. For pathogens, iron is a critical micronutrient as its bioavailability is usually low in bacterial niches. This metal is involved in critical physiological processes mainly as a component of important metabolic molecules involved in redox reactions. Usually bacteria respond to fluctuations in iron availability to regulate iron acquisition and other iron-related functions. Recently the close metabolic feedback between iron and riboflavin, another pivotal biological redox agent, began to draw attention in bacteria. This is a widespread biological phenomenon, partly characterized by the coordination of regulatory responses to iron and riboflavin, probably owed to the involvement of these cofactors in common processes. Nonetheless, no systematic analyses to determine the extent of this regulatory effect have been performed in any species. Here, the transcriptomics responses to iron, riboflavin, iron in the presence of riboflavin and riboflavin in the presence of iron were assessed and compared in S. flexneri. The riboflavin regulon had a 43% overlap with the iron regulon. Notably, the presence of riboflavin highly increased the number of iron-responsive genes. Reciprocally, iron drastically changed the pool of riboflavin-responsive genes. Gene ontology (GO) functional terms enrichment analysis showed that biological processes were distinctively enriched for each subgroup of responsive genes. Among the biological processes regulated by iron and riboflavin were iron uptake, amino acids metabolism and electron transfer for ATP synthesis. Thus, iron and riboflavin highly affect the transcriptomics responses induced by each other in S. flexneri. GO terms analysis suggests that iron and riboflavin coordinately regulate specific physiological functions involving redox metabolism. PeerJ Inc. 2020-09-15 /pmc/articles/PMC7500357/ /pubmed/32983629 http://dx.doi.org/10.7717/peerj.9553 Text en ©2020 Lozano Aguirre et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Genomics
Lozano Aguirre, Luis Fernando
Salazar, Juan Carlos
Vásquez, José Ignacio
García-Angulo, Víctor Antonio
Interdependency of regulatory effects of iron and riboflavin in the foodborne pathogen Shigella flexneri determined by integral transcriptomics
title Interdependency of regulatory effects of iron and riboflavin in the foodborne pathogen Shigella flexneri determined by integral transcriptomics
title_full Interdependency of regulatory effects of iron and riboflavin in the foodborne pathogen Shigella flexneri determined by integral transcriptomics
title_fullStr Interdependency of regulatory effects of iron and riboflavin in the foodborne pathogen Shigella flexneri determined by integral transcriptomics
title_full_unstemmed Interdependency of regulatory effects of iron and riboflavin in the foodborne pathogen Shigella flexneri determined by integral transcriptomics
title_short Interdependency of regulatory effects of iron and riboflavin in the foodborne pathogen Shigella flexneri determined by integral transcriptomics
title_sort interdependency of regulatory effects of iron and riboflavin in the foodborne pathogen shigella flexneri determined by integral transcriptomics
topic Genomics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7500357/
https://www.ncbi.nlm.nih.gov/pubmed/32983629
http://dx.doi.org/10.7717/peerj.9553
work_keys_str_mv AT lozanoaguirreluisfernando interdependencyofregulatoryeffectsofironandriboflavininthefoodbornepathogenshigellaflexnerideterminedbyintegraltranscriptomics
AT salazarjuancarlos interdependencyofregulatoryeffectsofironandriboflavininthefoodbornepathogenshigellaflexnerideterminedbyintegraltranscriptomics
AT vasquezjoseignacio interdependencyofregulatoryeffectsofironandriboflavininthefoodbornepathogenshigellaflexnerideterminedbyintegraltranscriptomics
AT garciaangulovictorantonio interdependencyofregulatoryeffectsofironandriboflavininthefoodbornepathogenshigellaflexnerideterminedbyintegraltranscriptomics