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Application of a cost-effective DNA extraction protocol for screening transgenic and CRISPR-edited primary goat cells

The genotyping of genetically-modified cells is a crucial step in studies of transgenics and genomic editing with systems such as CRISPR/Cas. The detection of genome editing events can be directly related to the genotyping methodology used, which is influenced by its costs, since many experiments re...

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Detalles Bibliográficos
Autores principales: Teixeira, Louhanna Pinheiro Rodrigues, Lopes, Francisco Eder de Moura, Antunes, André Saraiva Leão Marcelo, Alves, Matheus Soares, Miranda, André Marrocos, Gaudencio Neto, Saul, Martins, Leonardo Tondello, Moreira, Ana Cristina de Oliveira Monteiro, Tavares, Kaio Cesar Simiano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7500585/
https://www.ncbi.nlm.nih.gov/pubmed/32946490
http://dx.doi.org/10.1371/journal.pone.0239435