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Fluorescence Lifetime and Intensity of Thioflavin T as Reporters of Different Fibrillation Stages: Insights Obtained from Fluorescence Up-Conversion and Particle Size Distribution Measurements

Thioflavin T (ThT) assay is extensively used for studying fibrillation kinetics in vitro. However, the differences in the time course of ThT fluorescence intensity and lifetime and other physical parameters of the system, such as particle size distribution, raise questions about the correct interpre...

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Detalles Bibliográficos
Autores principales: Rovnyagina, Nataliya R., Budylin, Gleb S., Vainer, Yuri G., Tikhonova, Tatiana N., Vasin, Sergey L., Yakovlev, Alexander A., Kompanets, Victor O., Chekalin, Sergey V., Priezzhev, Alexander V., Shirshin, Evgeny A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504639/
https://www.ncbi.nlm.nih.gov/pubmed/32859090
http://dx.doi.org/10.3390/ijms21176169