Peel-1 negative selection promotes screening-free CRISPR-Cas9 genome editing in Caenorhabditis elegans

Improved genome engineering methods that enable automation of large and precise edits are essential for systematic investigations of genome function. We adapted peel-1 negative selection to an optimized Dual-Marker Selection (DMS) cassette protocol for CRISPR-Cas9 genome engineering in Caenorhabditi...

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Detalles Bibliográficos
Autores principales: McDiarmid, Troy A., Au, Vinci, Moerman, Donald G., Rankin, Catharine H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7508457/
https://www.ncbi.nlm.nih.gov/pubmed/32960926
http://dx.doi.org/10.1371/journal.pone.0238950

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7508457/
https://www.ncbi.nlm.nih.gov/pubmed/32960926
http://dx.doi.org/10.1371/journal.pone.0238950

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