Cas12a mediates efficient and precise endogenous gene tagging via MITI: microhomology-dependent targeted integrations

Efficient exogenous DNA integration can be mediated by Cas9 through the non-homology end-joining pathway. However, such integrations are often imprecise and contain a variety of mutations at the junctions between the external DNA and the genomic loci. Here we describe a microhomology-dependent targe...

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Detalles Bibliográficos
Autores principales: Li, Pan, Zhang, Lijun, Li, Zhifang, Xu, Chunlong, Du, Xuguang, Wu, Sen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2019
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7508734/
https://www.ncbi.nlm.nih.gov/pubmed/31848638
http://dx.doi.org/10.1007/s00018-019-03396-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7508734/
https://www.ncbi.nlm.nih.gov/pubmed/31848638
http://dx.doi.org/10.1007/s00018-019-03396-8

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