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Cas12a mediates efficient and precise endogenous gene tagging via MITI: microhomology-dependent targeted integrations
Efficient exogenous DNA integration can be mediated by Cas9 through the non-homology end-joining pathway. However, such integrations are often imprecise and contain a variety of mutations at the junctions between the external DNA and the genomic loci. Here we describe a microhomology-dependent targe...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer International Publishing
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7508734/ https://www.ncbi.nlm.nih.gov/pubmed/31848638 http://dx.doi.org/10.1007/s00018-019-03396-8 |