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Single-Chain Lanthanide Luminescence Biosensors for Cell-Based Imaging and Screening of Protein-Protein Interactions

Lanthanide-based, Förster resonance energy transfer (LRET) biosensors enabled sensitive, time-gated luminescence (TGL) imaging or multiwell plate analysis of protein-protein interactions (PPIs) in living cells. We prepared stable cell lines that expressed polypeptides composed of an alpha helical li...

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Detalles Bibliográficos
Autores principales: Chen, Ting, Pham, Ha, Mohamadi, Ali, Miller, Lawrence W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509216/
https://www.ncbi.nlm.nih.gov/pubmed/33083762
http://dx.doi.org/10.1016/j.isci.2020.101533