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Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2

We describe validated protocols for generating high-quality, full-length severe acute respiratory syndrome coronavirus 2 genomes from primary samples. One protocol uses multiplex reverse transcription PCR, followed by MinION or MiSeq sequencing; the other uses singleplex, nested reverse transcriptio...

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Detalles Bibliográficos
Autores principales: Paden, Clinton R., Tao, Ying, Queen, Krista, Zhang, Jing, Li, Yan, Uehara, Anna, Tong, Suxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510745/
https://www.ncbi.nlm.nih.gov/pubmed/32610037
http://dx.doi.org/10.3201/eid2610.201800