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RAP Tag and PMab-2 Antibody: A Tagging System for Detecting and Purifying Proteins in Plant Cells

An affinity tag system requires both high affinity and specificity. The RAP tag epitope DMVNPGLEDRIE, derived from rat podoplanin (PDPN), is specifically recognized by PMab-2 monoclonal antibodies in rats. Here, we demonstrated that high levels of PMab-2 can be produced in Nicotiana benthamiana and...

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Detalles Bibliográficos
Autores principales: Miura, Kenji, Yoshida, Hideki, Nosaki, Shohei, Kaneko, Mika K., Kato, Yukinari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511514/
https://www.ncbi.nlm.nih.gov/pubmed/33013955
http://dx.doi.org/10.3389/fpls.2020.510444