Ensemble-based enzyme design can recapitulate the effects of laboratory directed evolution in silico

The creation of artificial enzymes is a key objective of computational protein design. Although de novo enzymes have been successfully designed, these exhibit low catalytic efficiencies, requiring directed evolution to improve activity. Here, we use room-temperature X-ray crystallography to study ch...

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Detalles Bibliográficos
Autores principales: Broom, Aron, Rakotoharisoa, Rojo V., Thompson, Michael C., Zarifi, Niayesh, Nguyen, Erin, Mukhametzhanov, Nurzhan, Liu, Lin, Fraser, James S., Chica, Roberto A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511930/
https://www.ncbi.nlm.nih.gov/pubmed/32968058
http://dx.doi.org/10.1038/s41467-020-18619-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511930/
https://www.ncbi.nlm.nih.gov/pubmed/32968058
http://dx.doi.org/10.1038/s41467-020-18619-x

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