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Generation of Comprehensive Ecosystem-Specific Reference Databases with Species-Level Resolution by High-Throughput Full-Length 16S rRNA Gene Sequencing and Automated Taxonomy Assignment (AutoTax)

High-throughput 16S rRNA gene amplicon sequencing is an essential method for studying the diversity and dynamics of microbial communities. However, this method is presently hampered by the lack of high-identity reference sequences for many environmental microbes in the public 16S rRNA gene reference...

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Detalles Bibliográficos
Autores principales: Dueholm, Morten Simonsen, Andersen, Kasper Skytte, McIlroy, Simon Jon, Kristensen, Jannie Munk, Yashiro, Erika, Karst, Søren Michael, Albertsen, Mads, Nielsen, Per Halkjær
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7512547/
https://www.ncbi.nlm.nih.gov/pubmed/32963001
http://dx.doi.org/10.1128/mBio.01557-20