Evaluation of the In Vitro Stability of Stimuli-Sensitive Fatty Acid-Based Microparticles for the Treatment of Lung Cancer
[Image: see text] The fatty acid-based microparticles containing iron oxide nanoparticles and paclitaxel (PAX) are a viable proposition for the treatment of lung cancer. The microparticles inhaled as a dry powder can be guided to selected locations using an external magnetic field, and when accumula...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American
Chemical Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7513473/ https://www.ncbi.nlm.nih.gov/pubmed/32856922 http://dx.doi.org/10.1021/acs.langmuir.0c02141 |
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author | Dałek, Paulina Borowik, Tomasz Reczyńska, Katarzyna Pamuła, Elżbieta Chrzanowski, Wojciech Langner, Marek |
author_facet | Dałek, Paulina Borowik, Tomasz Reczyńska, Katarzyna Pamuła, Elżbieta Chrzanowski, Wojciech Langner, Marek |
author_sort | Dałek, Paulina |
collection | PubMed |
description | [Image: see text] The fatty acid-based microparticles containing iron oxide nanoparticles and paclitaxel (PAX) are a viable proposition for the treatment of lung cancer. The microparticles inhaled as a dry powder can be guided to selected locations using an external magnetic field, and when accumulated there, the active compound release can be triggered by local hyperthermia. However, this general strategy requires that the active compound is released from microparticles and can reach the targeted cells before microparticles are removed. Isothermal titration calorimetry was used to demonstrate that the components of microparticles were released and transferred to albumins and lipid bilayers. The morphology of the measured particulates was studied with scanning electron microscopy and dynamic light scattering. To determine the cytotoxicity of microparticles, cell culture studies were done. It has been shown that the transfer efficiency depends predominantly on the fatty acid composition of microparticles, which, together with the active ingredient, accumulate predominantly in membrane structures after being released from microparticles and before entering the cytoplasm. The release process is sufficient; hence, paclitaxel-loaded microparticles effectively suppressed the proliferation of A549 human lung epithelial cells of malignant origin (IC(50) values for both lauric acid-based and myristic/palmitic-based microparticles containing paclitaxel were below 0.375 μg/mL), while reference microparticles were noncytotoxic. |
format | Online Article Text |
id | pubmed-7513473 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American
Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-75134732020-09-25 Evaluation of the In Vitro Stability of Stimuli-Sensitive Fatty Acid-Based Microparticles for the Treatment of Lung Cancer Dałek, Paulina Borowik, Tomasz Reczyńska, Katarzyna Pamuła, Elżbieta Chrzanowski, Wojciech Langner, Marek Langmuir [Image: see text] The fatty acid-based microparticles containing iron oxide nanoparticles and paclitaxel (PAX) are a viable proposition for the treatment of lung cancer. The microparticles inhaled as a dry powder can be guided to selected locations using an external magnetic field, and when accumulated there, the active compound release can be triggered by local hyperthermia. However, this general strategy requires that the active compound is released from microparticles and can reach the targeted cells before microparticles are removed. Isothermal titration calorimetry was used to demonstrate that the components of microparticles were released and transferred to albumins and lipid bilayers. The morphology of the measured particulates was studied with scanning electron microscopy and dynamic light scattering. To determine the cytotoxicity of microparticles, cell culture studies were done. It has been shown that the transfer efficiency depends predominantly on the fatty acid composition of microparticles, which, together with the active ingredient, accumulate predominantly in membrane structures after being released from microparticles and before entering the cytoplasm. The release process is sufficient; hence, paclitaxel-loaded microparticles effectively suppressed the proliferation of A549 human lung epithelial cells of malignant origin (IC(50) values for both lauric acid-based and myristic/palmitic-based microparticles containing paclitaxel were below 0.375 μg/mL), while reference microparticles were noncytotoxic. American Chemical Society 2020-08-28 2020-09-22 /pmc/articles/PMC7513473/ /pubmed/32856922 http://dx.doi.org/10.1021/acs.langmuir.0c02141 Text en Copyright © 2020 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. |
spellingShingle | Dałek, Paulina Borowik, Tomasz Reczyńska, Katarzyna Pamuła, Elżbieta Chrzanowski, Wojciech Langner, Marek Evaluation of the In Vitro Stability of Stimuli-Sensitive Fatty Acid-Based Microparticles for the Treatment of Lung Cancer |
title | Evaluation of the In Vitro Stability of Stimuli-Sensitive
Fatty Acid-Based Microparticles for the Treatment of Lung Cancer |
title_full | Evaluation of the In Vitro Stability of Stimuli-Sensitive
Fatty Acid-Based Microparticles for the Treatment of Lung Cancer |
title_fullStr | Evaluation of the In Vitro Stability of Stimuli-Sensitive
Fatty Acid-Based Microparticles for the Treatment of Lung Cancer |
title_full_unstemmed | Evaluation of the In Vitro Stability of Stimuli-Sensitive
Fatty Acid-Based Microparticles for the Treatment of Lung Cancer |
title_short | Evaluation of the In Vitro Stability of Stimuli-Sensitive
Fatty Acid-Based Microparticles for the Treatment of Lung Cancer |
title_sort | evaluation of the in vitro stability of stimuli-sensitive
fatty acid-based microparticles for the treatment of lung cancer |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7513473/ https://www.ncbi.nlm.nih.gov/pubmed/32856922 http://dx.doi.org/10.1021/acs.langmuir.0c02141 |
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