Topical ripasudil stimulates neuroprotection and axon regeneration in adult mice following optic nerve injury

Optic nerve injury induces optic nerve degeneration and retinal ganglion cell (RGC) death that lead to visual disturbance. In this study, we examined if topical ripasudil has therapeutic potential in adult mice after optic nerve crush (ONC). Topical ripasudil suppressed ONC-induced phosphorylation o...

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Autores principales: Nishijima, Euido, Namekata, Kazuhiko, Kimura, Atsuko, Guo, Xiaoli, Harada, Chikako, Noro, Takahiko, Nakano, Tadashi, Harada, Takayuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7515881/
https://www.ncbi.nlm.nih.gov/pubmed/32973242
http://dx.doi.org/10.1038/s41598-020-72748-3
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author Nishijima, Euido
Namekata, Kazuhiko
Kimura, Atsuko
Guo, Xiaoli
Harada, Chikako
Noro, Takahiko
Nakano, Tadashi
Harada, Takayuki
author_facet Nishijima, Euido
Namekata, Kazuhiko
Kimura, Atsuko
Guo, Xiaoli
Harada, Chikako
Noro, Takahiko
Nakano, Tadashi
Harada, Takayuki
author_sort Nishijima, Euido
collection PubMed
description Optic nerve injury induces optic nerve degeneration and retinal ganglion cell (RGC) death that lead to visual disturbance. In this study, we examined if topical ripasudil has therapeutic potential in adult mice after optic nerve crush (ONC). Topical ripasudil suppressed ONC-induced phosphorylation of p38 mitogen-activated protein kinase and ameliorated RGC death. In addition, topical ripasudil significantly suppressed the phosphorylation of collapsin response mediator protein 2 and cofilin, and promoted optic nerve regeneration. These results suggest that topical ripasudil promotes RGC protection and optic nerve regeneration by modulating multiple signaling pathways associated with neural cell death, microtubule assembly and actin polymerization.
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spelling pubmed-75158812020-09-29 Topical ripasudil stimulates neuroprotection and axon regeneration in adult mice following optic nerve injury Nishijima, Euido Namekata, Kazuhiko Kimura, Atsuko Guo, Xiaoli Harada, Chikako Noro, Takahiko Nakano, Tadashi Harada, Takayuki Sci Rep Article Optic nerve injury induces optic nerve degeneration and retinal ganglion cell (RGC) death that lead to visual disturbance. In this study, we examined if topical ripasudil has therapeutic potential in adult mice after optic nerve crush (ONC). Topical ripasudil suppressed ONC-induced phosphorylation of p38 mitogen-activated protein kinase and ameliorated RGC death. In addition, topical ripasudil significantly suppressed the phosphorylation of collapsin response mediator protein 2 and cofilin, and promoted optic nerve regeneration. These results suggest that topical ripasudil promotes RGC protection and optic nerve regeneration by modulating multiple signaling pathways associated with neural cell death, microtubule assembly and actin polymerization. Nature Publishing Group UK 2020-09-24 /pmc/articles/PMC7515881/ /pubmed/32973242 http://dx.doi.org/10.1038/s41598-020-72748-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Nishijima, Euido
Namekata, Kazuhiko
Kimura, Atsuko
Guo, Xiaoli
Harada, Chikako
Noro, Takahiko
Nakano, Tadashi
Harada, Takayuki
Topical ripasudil stimulates neuroprotection and axon regeneration in adult mice following optic nerve injury
title Topical ripasudil stimulates neuroprotection and axon regeneration in adult mice following optic nerve injury
title_full Topical ripasudil stimulates neuroprotection and axon regeneration in adult mice following optic nerve injury
title_fullStr Topical ripasudil stimulates neuroprotection and axon regeneration in adult mice following optic nerve injury
title_full_unstemmed Topical ripasudil stimulates neuroprotection and axon regeneration in adult mice following optic nerve injury
title_short Topical ripasudil stimulates neuroprotection and axon regeneration in adult mice following optic nerve injury
title_sort topical ripasudil stimulates neuroprotection and axon regeneration in adult mice following optic nerve injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7515881/
https://www.ncbi.nlm.nih.gov/pubmed/32973242
http://dx.doi.org/10.1038/s41598-020-72748-3
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