pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110

The pSETT4 vector integrates into the Actinoplanes sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4 terminators surround the expression cassette to isolate the transcriptional unit and to prevent antisense transc...

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Detalles Bibliográficos
Autores principales: Schaffert, Lena, Jacob, Lucas, Schneiker-Bekel, Susanne, Persicke, Marcus, März, Camilla, Rückert, Christian, Pühler, Alfred, Kalinowski, Jörn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2020
Materias:
Other Genetic Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7516140/
https://www.ncbi.nlm.nih.gov/pubmed/32972929
http://dx.doi.org/10.1128/MRA.00596-20

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7516140/
https://www.ncbi.nlm.nih.gov/pubmed/32972929
http://dx.doi.org/10.1128/MRA.00596-20

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