pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110

The pSETT4 vector integrates into the Actinoplanes sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4 terminators surround the expression cassette to isolate the transcriptional unit and to prevent antisense transc...

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Detalles Bibliográficos
Autores principales: Schaffert, Lena, Jacob, Lucas, Schneiker-Bekel, Susanne, Persicke, Marcus, März, Camilla, Rückert, Christian, Pühler, Alfred, Kalinowski, Jörn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2020
Materias:
Other Genetic Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7516140/
https://www.ncbi.nlm.nih.gov/pubmed/32972929
http://dx.doi.org/10.1128/MRA.00596-20
Descripción
Sumario:The pSETT4 vector integrates into the Actinoplanes sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4 terminators surround the expression cassette to isolate the transcriptional unit and to prevent antisense transcription. The system can be used in other Actinomycetales by exchanging the promoter.

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