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pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110

The pSETT4 vector integrates into the Actinoplanes sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4 terminators surround the expression cassette to isolate the transcriptional unit and to prevent antisense transc...

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Detalles Bibliográficos
Autores principales: Schaffert, Lena, Jacob, Lucas, Schneiker-Bekel, Susanne, Persicke, Marcus, März, Camilla, Rückert, Christian, Pühler, Alfred, Kalinowski, Jörn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7516140/
https://www.ncbi.nlm.nih.gov/pubmed/32972929
http://dx.doi.org/10.1128/MRA.00596-20
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author Schaffert, Lena
Jacob, Lucas
Schneiker-Bekel, Susanne
Persicke, Marcus
März, Camilla
Rückert, Christian
Pühler, Alfred
Kalinowski, Jörn
author_facet Schaffert, Lena
Jacob, Lucas
Schneiker-Bekel, Susanne
Persicke, Marcus
März, Camilla
Rückert, Christian
Pühler, Alfred
Kalinowski, Jörn
author_sort Schaffert, Lena
collection PubMed
description The pSETT4 vector integrates into the Actinoplanes sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4 terminators surround the expression cassette to isolate the transcriptional unit and to prevent antisense transcription. The system can be used in other Actinomycetales by exchanging the promoter.
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spelling pubmed-75161402020-10-02 pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110 Schaffert, Lena Jacob, Lucas Schneiker-Bekel, Susanne Persicke, Marcus März, Camilla Rückert, Christian Pühler, Alfred Kalinowski, Jörn Microbiol Resour Announc Other Genetic Resources The pSETT4 vector integrates into the Actinoplanes sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4 terminators surround the expression cassette to isolate the transcriptional unit and to prevent antisense transcription. The system can be used in other Actinomycetales by exchanging the promoter. American Society for Microbiology 2020-09-24 /pmc/articles/PMC7516140/ /pubmed/32972929 http://dx.doi.org/10.1128/MRA.00596-20 Text en Copyright © 2020 Schaffert et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Other Genetic Resources
Schaffert, Lena
Jacob, Lucas
Schneiker-Bekel, Susanne
Persicke, Marcus
März, Camilla
Rückert, Christian
Pühler, Alfred
Kalinowski, Jörn
pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110
title pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110
title_full pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110
title_fullStr pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110
title_full_unstemmed pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110
title_short pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110
title_sort psett4, an improved φc31-based integrative vector system for actinoplanes sp. se50/110
topic Other Genetic Resources
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7516140/
https://www.ncbi.nlm.nih.gov/pubmed/32972929
http://dx.doi.org/10.1128/MRA.00596-20
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