Cargando…
pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110
The pSETT4 vector integrates into the Actinoplanes sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4 terminators surround the expression cassette to isolate the transcriptional unit and to prevent antisense transc...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7516140/ https://www.ncbi.nlm.nih.gov/pubmed/32972929 http://dx.doi.org/10.1128/MRA.00596-20 |
_version_ | 1783586942224433152 |
---|---|
author | Schaffert, Lena Jacob, Lucas Schneiker-Bekel, Susanne Persicke, Marcus März, Camilla Rückert, Christian Pühler, Alfred Kalinowski, Jörn |
author_facet | Schaffert, Lena Jacob, Lucas Schneiker-Bekel, Susanne Persicke, Marcus März, Camilla Rückert, Christian Pühler, Alfred Kalinowski, Jörn |
author_sort | Schaffert, Lena |
collection | PubMed |
description | The pSETT4 vector integrates into the Actinoplanes sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4 terminators surround the expression cassette to isolate the transcriptional unit and to prevent antisense transcription. The system can be used in other Actinomycetales by exchanging the promoter. |
format | Online Article Text |
id | pubmed-7516140 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-75161402020-10-02 pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110 Schaffert, Lena Jacob, Lucas Schneiker-Bekel, Susanne Persicke, Marcus März, Camilla Rückert, Christian Pühler, Alfred Kalinowski, Jörn Microbiol Resour Announc Other Genetic Resources The pSETT4 vector integrates into the Actinoplanes sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4 terminators surround the expression cassette to isolate the transcriptional unit and to prevent antisense transcription. The system can be used in other Actinomycetales by exchanging the promoter. American Society for Microbiology 2020-09-24 /pmc/articles/PMC7516140/ /pubmed/32972929 http://dx.doi.org/10.1128/MRA.00596-20 Text en Copyright © 2020 Schaffert et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Other Genetic Resources Schaffert, Lena Jacob, Lucas Schneiker-Bekel, Susanne Persicke, Marcus März, Camilla Rückert, Christian Pühler, Alfred Kalinowski, Jörn pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110 |
title | pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110 |
title_full | pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110 |
title_fullStr | pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110 |
title_full_unstemmed | pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110 |
title_short | pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110 |
title_sort | psett4, an improved φc31-based integrative vector system for actinoplanes sp. se50/110 |
topic | Other Genetic Resources |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7516140/ https://www.ncbi.nlm.nih.gov/pubmed/32972929 http://dx.doi.org/10.1128/MRA.00596-20 |
work_keys_str_mv | AT schaffertlena psett4animprovedphc31basedintegrativevectorsystemforactinoplanesspse50110 AT jacoblucas psett4animprovedphc31basedintegrativevectorsystemforactinoplanesspse50110 AT schneikerbekelsusanne psett4animprovedphc31basedintegrativevectorsystemforactinoplanesspse50110 AT persickemarcus psett4animprovedphc31basedintegrativevectorsystemforactinoplanesspse50110 AT marzcamilla psett4animprovedphc31basedintegrativevectorsystemforactinoplanesspse50110 AT ruckertchristian psett4animprovedphc31basedintegrativevectorsystemforactinoplanesspse50110 AT puhleralfred psett4animprovedphc31basedintegrativevectorsystemforactinoplanesspse50110 AT kalinowskijorn psett4animprovedphc31basedintegrativevectorsystemforactinoplanesspse50110 |