Protocol for Comprehensive Synthetic Lethality Screens

Here, we provide a detailed protocol for synthetic lethality screens in a Jurkat T cell leukemia line using cell death as the readout measuring the combinatorial effect of a pan-PI3K inhibitor (GDC0941) with specific gene depletion by shRNA. We describe the use of an ultra-complex shRNA library, cov...

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Detalles Bibliográficos
Autores principales: Romero-Moya, Damia, Roose, Jeroen P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7518629/
https://www.ncbi.nlm.nih.gov/pubmed/32984851
http://dx.doi.org/10.1016/j.xpro.2020.100016

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7518629/
https://www.ncbi.nlm.nih.gov/pubmed/32984851
http://dx.doi.org/10.1016/j.xpro.2020.100016

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