Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing

Precision genome engineering has dramatically advanced with the development of CRISPR/Cas base editing systems that include cytosine base editors and adenine base editors (ABEs). Herein, we compare the editing profile of circularly permuted and domain-inlaid Cas9 base editors, and find that on-targe...

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Detalles Bibliográficos
Autores principales: Nguyen Tran, Minh Thuan, Mohd Khalid, Mohd Khairul Nizam, Wang, Qi, Walker, Jacqueline K. R., Lidgerwood, Grace E., Dilworth, Kimberley L., Lisowski, Leszek, Pébay, Alice, Hewitt, Alex W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7519688/
https://www.ncbi.nlm.nih.gov/pubmed/32978399
http://dx.doi.org/10.1038/s41467-020-18715-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7519688/
https://www.ncbi.nlm.nih.gov/pubmed/32978399
http://dx.doi.org/10.1038/s41467-020-18715-y

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