Knocking out Analysis of the CpxP gene using Crispr/Cas9 in Escherichia coli MG1655

Based on the analysis of cpxP genes among Escherichia coli strains, cpxP gene-targeting short guide RNA (sgRNA) was designed and inserted into the pGL3-MGP-RNA. The donor sequences (MG-HR) for homologous repair were designed and cloned by PCR. MG-HR and pGL3-MGP-RNA were transformed into E. coli MG1...

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Detalles Bibliográficos
Autores principales: He, Xiaoliang, Ren, Yuwen, Meng, Wanli, Yu, Xinran, Zhou, Xiaohui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7519928/
https://www.ncbi.nlm.nih.gov/pubmed/32979149
http://dx.doi.org/10.1186/s13568-020-01099-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7519928/
https://www.ncbi.nlm.nih.gov/pubmed/32979149
http://dx.doi.org/10.1186/s13568-020-01099-z

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