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Curcumin and hesperetin attenuate D-galactose-induced brain senescence in vitro and in vivo

BACKGROUND/OBJECTIVES: Brain senescence causes cognitive impairment and neurodegeneration. It has also been demonstrated that curcumin (Cur) and hesperetin (Hes), both antioxidant polyphenolic compounds, mediate anti-aging and neuroprotective effects. Therefore, the objective of this study was to in...

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Autores principales: Lee, Jihye, Kim, Yoo Sun, Kim, Eunju, Kim, Yerin, Kim, Yuri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Nutrition Society and the Korean Society of Community Nutrition 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7520561/
https://www.ncbi.nlm.nih.gov/pubmed/33029285
http://dx.doi.org/10.4162/nrp.2020.14.5.438
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author Lee, Jihye
Kim, Yoo Sun
Kim, Eunju
Kim, Yerin
Kim, Yuri
author_facet Lee, Jihye
Kim, Yoo Sun
Kim, Eunju
Kim, Yerin
Kim, Yuri
author_sort Lee, Jihye
collection PubMed
description BACKGROUND/OBJECTIVES: Brain senescence causes cognitive impairment and neurodegeneration. It has also been demonstrated that curcumin (Cur) and hesperetin (Hes), both antioxidant polyphenolic compounds, mediate anti-aging and neuroprotective effects. Therefore, the objective of this study was to investigate whether Cur, Hes, and/or their combination exert anti-aging effects in D-galactose (Dg)-induced aged neuronal cells and rats. MATERIALS/METHODS: SH-SY5Y cells differentiated in response to retinoic acid were treated with Cur (1 μM), Hes (1 μM), or a combination of both, followed by 300 mM Dg. Neuronal loss was subsequently evaluated by measuring average neurite length and analyzing expression of β-tubulin III, phosphorylated extracellular signal-regulated kinases, and neurofilament heavy polypeptide. Cellular senescence and related proteins, p16 and p21, were also investigated, including their regulation of antioxidant enzymes. In vivo, brain aging was induced by injecting 250 mg/kg body weight (b.w.) Dg. The effects of supplementing this model with 50 mg/kg b.w. Cur, 50 mg/kg b.w. Hes, or a combination of both for 3 months were subsequently evaluated. Brain aging was examined with a step-through passive avoidance test and apoptosis markers were analyzed in brain cortex tissues. RESULTS: Cur, Hes, and their combination improved neuron length and cellular senescence by decreasing the number of β-gal stained cells, down-regulated expression of p16 and p21, and up-regulated expression of antioxidant enzymes, including superoxide dismutase 1, glutathione peroxidase 1, and catalase. Administration of Cur, Hes, or their combination also tended to ameliorate cognitive impairment and suppress apoptosis in the cerebral cortex by down-regulating Bax and poly (ADP-ribose) polymerase expression and increasing Bcl-2 expression. CONCLUSIONS: Cur and Hes appear to attenuate Dg-induced brain aging via regulation of antioxidant enzymes and apoptosis. These results suggest that Cur and Hes may mediate neuroprotective effects in the aging process, and further study of these antioxidant polyphenolic compounds is warranted.
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spelling pubmed-75205612020-10-06 Curcumin and hesperetin attenuate D-galactose-induced brain senescence in vitro and in vivo Lee, Jihye Kim, Yoo Sun Kim, Eunju Kim, Yerin Kim, Yuri Nutr Res Pract Original Research BACKGROUND/OBJECTIVES: Brain senescence causes cognitive impairment and neurodegeneration. It has also been demonstrated that curcumin (Cur) and hesperetin (Hes), both antioxidant polyphenolic compounds, mediate anti-aging and neuroprotective effects. Therefore, the objective of this study was to investigate whether Cur, Hes, and/or their combination exert anti-aging effects in D-galactose (Dg)-induced aged neuronal cells and rats. MATERIALS/METHODS: SH-SY5Y cells differentiated in response to retinoic acid were treated with Cur (1 μM), Hes (1 μM), or a combination of both, followed by 300 mM Dg. Neuronal loss was subsequently evaluated by measuring average neurite length and analyzing expression of β-tubulin III, phosphorylated extracellular signal-regulated kinases, and neurofilament heavy polypeptide. Cellular senescence and related proteins, p16 and p21, were also investigated, including their regulation of antioxidant enzymes. In vivo, brain aging was induced by injecting 250 mg/kg body weight (b.w.) Dg. The effects of supplementing this model with 50 mg/kg b.w. Cur, 50 mg/kg b.w. Hes, or a combination of both for 3 months were subsequently evaluated. Brain aging was examined with a step-through passive avoidance test and apoptosis markers were analyzed in brain cortex tissues. RESULTS: Cur, Hes, and their combination improved neuron length and cellular senescence by decreasing the number of β-gal stained cells, down-regulated expression of p16 and p21, and up-regulated expression of antioxidant enzymes, including superoxide dismutase 1, glutathione peroxidase 1, and catalase. Administration of Cur, Hes, or their combination also tended to ameliorate cognitive impairment and suppress apoptosis in the cerebral cortex by down-regulating Bax and poly (ADP-ribose) polymerase expression and increasing Bcl-2 expression. CONCLUSIONS: Cur and Hes appear to attenuate Dg-induced brain aging via regulation of antioxidant enzymes and apoptosis. These results suggest that Cur and Hes may mediate neuroprotective effects in the aging process, and further study of these antioxidant polyphenolic compounds is warranted. The Korean Nutrition Society and the Korean Society of Community Nutrition 2020-10 2020-08-06 /pmc/articles/PMC7520561/ /pubmed/33029285 http://dx.doi.org/10.4162/nrp.2020.14.5.438 Text en ©2020 The Korean Nutrition Society and the Korean Society of Community Nutrition https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Lee, Jihye
Kim, Yoo Sun
Kim, Eunju
Kim, Yerin
Kim, Yuri
Curcumin and hesperetin attenuate D-galactose-induced brain senescence in vitro and in vivo
title Curcumin and hesperetin attenuate D-galactose-induced brain senescence in vitro and in vivo
title_full Curcumin and hesperetin attenuate D-galactose-induced brain senescence in vitro and in vivo
title_fullStr Curcumin and hesperetin attenuate D-galactose-induced brain senescence in vitro and in vivo
title_full_unstemmed Curcumin and hesperetin attenuate D-galactose-induced brain senescence in vitro and in vivo
title_short Curcumin and hesperetin attenuate D-galactose-induced brain senescence in vitro and in vivo
title_sort curcumin and hesperetin attenuate d-galactose-induced brain senescence in vitro and in vivo
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7520561/
https://www.ncbi.nlm.nih.gov/pubmed/33029285
http://dx.doi.org/10.4162/nrp.2020.14.5.438
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