Protocol for the Analysis of Yeast and Human Mitochondrial Respiratory Chain Complexes and Supercomplexes by Blue Native Electrophoresis

By using negatively charged Coomassie brilliant blue G-250 dye to induce a charge shift on proteins, blue native polyacrylamide gel electrophoresis (BN-PAGE) allows resolution of enzymatically active multiprotein complexes extracted from cellular or subcellular lysates while retaining their native c...

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Detalles Bibliográficos
Autores principales: Timón-Gómez, Alba, Pérez-Pérez, Rafael, Nyvltova, Eva, Ugalde, Cristina, Fontanesi, Flavia, Barrientos, Antoni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521667/
https://www.ncbi.nlm.nih.gov/pubmed/32995753
http://dx.doi.org/10.1016/j.xpro.2020.100089

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521667/
https://www.ncbi.nlm.nih.gov/pubmed/32995753
http://dx.doi.org/10.1016/j.xpro.2020.100089

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