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Versatile in vitro assay to recognize Cas9‐induced mutations

The discovery of CRISPR/Cas9 has revolutionized molecular biology, and its impact on plant biotechnology and plant breeding cannot be over‐estimated. In many plant species, its application for mutagenesis is now a routine procedure––if suitable target sites, sufficient expression of the Cas9 protein...

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Detalles Bibliográficos
Autores principales: Bente, Heinrich, Mittelsten Scheid, Ortrun, Donà, Mattia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7522499/
https://www.ncbi.nlm.nih.gov/pubmed/33015536
http://dx.doi.org/10.1002/pld3.269