Sensitivity of shotgun metagenomics to host DNA: abundance estimates depend on bioinformatic tools and contamination is the main issue

A recent study reported that increasing host DNA abundance and reducing read depth impairs the sensitivity of detection of low-abundance micro-organisms by shotgun metagenomics. The authors used DNA from a synthetic bacterial community with abundances varying across several orders of magnitude and a...

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Detalles Bibliográficos
Autores principales: McArdle, Andrew J., Kaforou, Myrsini
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2020
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523627/
https://www.ncbi.nlm.nih.gov/pubmed/33005868
http://dx.doi.org/10.1099/acmi.0.000104

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523627/
https://www.ncbi.nlm.nih.gov/pubmed/33005868
http://dx.doi.org/10.1099/acmi.0.000104

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