Cargando…

Efficacy of Herbmed Plus in urolithic rats: An experimental study

BACKGROUND: In Ayurveda, several herbs and formulations are available for the treatment of Urolithiasis. However, they are not systematically evaluated for their safety, efficacy, indication and limitations. Herbmed Plus is one such herbal formulation that has been known for the management of urinar...

Descripción completa

Detalles Bibliográficos
Autores principales: Patankar, Suresh, Fanthome, Bernard, Bhalerao, Sagar S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7527796/
https://www.ncbi.nlm.nih.gov/pubmed/32241632
http://dx.doi.org/10.1016/j.jaim.2019.09.007
_version_ 1783589133843693568
author Patankar, Suresh
Fanthome, Bernard
Bhalerao, Sagar S.
author_facet Patankar, Suresh
Fanthome, Bernard
Bhalerao, Sagar S.
author_sort Patankar, Suresh
collection PubMed
description BACKGROUND: In Ayurveda, several herbs and formulations are available for the treatment of Urolithiasis. However, they are not systematically evaluated for their safety, efficacy, indication and limitations. Herbmed Plus is one such herbal formulation that has been known for the management of urinary tract disorders. An attempt has been made to evaluate its efficacy on Urolithiasis. OBJECTIVE: To evaluate the efficacy and safety of Herbmed Plus in urolithic rats. MATERIALS AND METHODS: A total of 60 Wistar albino rats were used for this study. The male and female rats were divided into five groups: disease control, test (dose 90 mg/kg), standard I (Cystone), standard II (Alkaston insta) and normal control (six in each group). Urolithiasis was induced using ethylene glycol 0.75% in drinking water for 28 days. The rats with urinary oxalate crystals were dosed with oral test or standard treatments for 28 days. RESULTS: All the animals appeared normal and showed no clinical signs of toxicity. None of the groups reported mortality or adverse effect on body weight and food consumption. The treatment with test drug showed improvement in the SGPT level and urine output (5.4 vs 3.47 mL/24 h). A drastic reduction in number of crystals were observed in male 0.5 vs 22 and female rats 0 vs 22.7 in test and disease group. The kidney lactate dehydrogenase, alkaline phosphatase, urinary phosphorus and calcium oxalate level decreased in the test and standard drug groups as compared to disease groups. Microscopy of the urine samples showed reduction in the number of crystals after treatment compared to the urolithic group. Increase in citrate levels in urine in all the treatment groups indicated anti-urolithiatic activity. The test group showed a 69.70% recovery in males and 47.57% recovery in female rats compared to the disease control group. CONCLUSION: Herbmed Plus showed a significant reduction in oxalate synthesizing enzymes suggesting anti-urolithiatic activity and anti-inflammatory and regenerative property in cellular injury caused by crystal deposits.
format Online
Article
Text
id pubmed-7527796
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-75277962020-10-05 Efficacy of Herbmed Plus in urolithic rats: An experimental study Patankar, Suresh Fanthome, Bernard Bhalerao, Sagar S. J Ayurveda Integr Med Original Research Article (Experimental) BACKGROUND: In Ayurveda, several herbs and formulations are available for the treatment of Urolithiasis. However, they are not systematically evaluated for their safety, efficacy, indication and limitations. Herbmed Plus is one such herbal formulation that has been known for the management of urinary tract disorders. An attempt has been made to evaluate its efficacy on Urolithiasis. OBJECTIVE: To evaluate the efficacy and safety of Herbmed Plus in urolithic rats. MATERIALS AND METHODS: A total of 60 Wistar albino rats were used for this study. The male and female rats were divided into five groups: disease control, test (dose 90 mg/kg), standard I (Cystone), standard II (Alkaston insta) and normal control (six in each group). Urolithiasis was induced using ethylene glycol 0.75% in drinking water for 28 days. The rats with urinary oxalate crystals were dosed with oral test or standard treatments for 28 days. RESULTS: All the animals appeared normal and showed no clinical signs of toxicity. None of the groups reported mortality or adverse effect on body weight and food consumption. The treatment with test drug showed improvement in the SGPT level and urine output (5.4 vs 3.47 mL/24 h). A drastic reduction in number of crystals were observed in male 0.5 vs 22 and female rats 0 vs 22.7 in test and disease group. The kidney lactate dehydrogenase, alkaline phosphatase, urinary phosphorus and calcium oxalate level decreased in the test and standard drug groups as compared to disease groups. Microscopy of the urine samples showed reduction in the number of crystals after treatment compared to the urolithic group. Increase in citrate levels in urine in all the treatment groups indicated anti-urolithiatic activity. The test group showed a 69.70% recovery in males and 47.57% recovery in female rats compared to the disease control group. CONCLUSION: Herbmed Plus showed a significant reduction in oxalate synthesizing enzymes suggesting anti-urolithiatic activity and anti-inflammatory and regenerative property in cellular injury caused by crystal deposits. Elsevier 2020 2020-03-30 /pmc/articles/PMC7527796/ /pubmed/32241632 http://dx.doi.org/10.1016/j.jaim.2019.09.007 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Research Article (Experimental)
Patankar, Suresh
Fanthome, Bernard
Bhalerao, Sagar S.
Efficacy of Herbmed Plus in urolithic rats: An experimental study
title Efficacy of Herbmed Plus in urolithic rats: An experimental study
title_full Efficacy of Herbmed Plus in urolithic rats: An experimental study
title_fullStr Efficacy of Herbmed Plus in urolithic rats: An experimental study
title_full_unstemmed Efficacy of Herbmed Plus in urolithic rats: An experimental study
title_short Efficacy of Herbmed Plus in urolithic rats: An experimental study
title_sort efficacy of herbmed plus in urolithic rats: an experimental study
topic Original Research Article (Experimental)
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7527796/
https://www.ncbi.nlm.nih.gov/pubmed/32241632
http://dx.doi.org/10.1016/j.jaim.2019.09.007
work_keys_str_mv AT patankarsuresh efficacyofherbmedplusinurolithicratsanexperimentalstudy
AT fanthomebernard efficacyofherbmedplusinurolithicratsanexperimentalstudy
AT bhaleraosagars efficacyofherbmedplusinurolithicratsanexperimentalstudy