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Cucurbitacin D Induces G2/M Phase Arrest and Apoptosis via the ROS/p38 Pathway in Capan-1 Pancreatic Cancer Cell Line

Pancreatic cancer has a poor prognosis with a five-year survival rate of less than 10%. Moreover, chemotherapy is mostly rendered ineffective owing to chemotherapy resistance and cytotoxicity. Therefore, the development of effective therapeutic strategies and novel drugs against pancreatic cancer is...

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Autores principales: Kim, Myeong-Sun, Lee, Kangwook, Ku, Jin Mo, Choi, Yu-Jeong, Mok, Kyungyul, Kim, Doori, Cheon, Chunhoo, Ko, Seong-Gyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7527894/
https://www.ncbi.nlm.nih.gov/pubmed/33029168
http://dx.doi.org/10.1155/2020/6571674
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author Kim, Myeong-Sun
Lee, Kangwook
Ku, Jin Mo
Choi, Yu-Jeong
Mok, Kyungyul
Kim, Doori
Cheon, Chunhoo
Ko, Seong-Gyu
author_facet Kim, Myeong-Sun
Lee, Kangwook
Ku, Jin Mo
Choi, Yu-Jeong
Mok, Kyungyul
Kim, Doori
Cheon, Chunhoo
Ko, Seong-Gyu
author_sort Kim, Myeong-Sun
collection PubMed
description Pancreatic cancer has a poor prognosis with a five-year survival rate of less than 10%. Moreover, chemotherapy is mostly rendered ineffective owing to chemotherapy resistance and cytotoxicity. Therefore, the development of effective therapeutic strategies and novel drugs against pancreatic cancer is an urgent need. Cucurbitacin D (CuD), a plant steroid derived from Trichosanthes kirilowii, is an anticancer agent effective against various cancer cell lines. However, the anticancer activity and molecular mechanism of CuD in pancreatic cancer remain unknown. Therefore, we aimed to investigate the anticancer activity and molecular mechanism of CuD in the human pancreatic cancer cell line, Capan-1. CuD induced cell cycle arrest at the G2/M phase, apoptosis, and reactive oxygen species generation in Capan-1 cell line. In addition, CuD induced the activation of the p38 MAPK signaling pathway that regulates apoptosis, which was also inhibited by N-acetyl-L-cysteine and the p38 inhibitor SB203580. These data suggest that CuD induces cell cycle arrest and apoptosis via the ROS/p38 pathway in Capan-1 pancreatic cancer cell line; hence, CuD is a promising candidate that should be explored further for its effectiveness as an anticancer agent against pancreatic cancer.
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spelling pubmed-75278942020-10-06 Cucurbitacin D Induces G2/M Phase Arrest and Apoptosis via the ROS/p38 Pathway in Capan-1 Pancreatic Cancer Cell Line Kim, Myeong-Sun Lee, Kangwook Ku, Jin Mo Choi, Yu-Jeong Mok, Kyungyul Kim, Doori Cheon, Chunhoo Ko, Seong-Gyu Evid Based Complement Alternat Med Research Article Pancreatic cancer has a poor prognosis with a five-year survival rate of less than 10%. Moreover, chemotherapy is mostly rendered ineffective owing to chemotherapy resistance and cytotoxicity. Therefore, the development of effective therapeutic strategies and novel drugs against pancreatic cancer is an urgent need. Cucurbitacin D (CuD), a plant steroid derived from Trichosanthes kirilowii, is an anticancer agent effective against various cancer cell lines. However, the anticancer activity and molecular mechanism of CuD in pancreatic cancer remain unknown. Therefore, we aimed to investigate the anticancer activity and molecular mechanism of CuD in the human pancreatic cancer cell line, Capan-1. CuD induced cell cycle arrest at the G2/M phase, apoptosis, and reactive oxygen species generation in Capan-1 cell line. In addition, CuD induced the activation of the p38 MAPK signaling pathway that regulates apoptosis, which was also inhibited by N-acetyl-L-cysteine and the p38 inhibitor SB203580. These data suggest that CuD induces cell cycle arrest and apoptosis via the ROS/p38 pathway in Capan-1 pancreatic cancer cell line; hence, CuD is a promising candidate that should be explored further for its effectiveness as an anticancer agent against pancreatic cancer. Hindawi 2020-09-22 /pmc/articles/PMC7527894/ /pubmed/33029168 http://dx.doi.org/10.1155/2020/6571674 Text en Copyright © 2020 Myeong-Sun Kim et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kim, Myeong-Sun
Lee, Kangwook
Ku, Jin Mo
Choi, Yu-Jeong
Mok, Kyungyul
Kim, Doori
Cheon, Chunhoo
Ko, Seong-Gyu
Cucurbitacin D Induces G2/M Phase Arrest and Apoptosis via the ROS/p38 Pathway in Capan-1 Pancreatic Cancer Cell Line
title Cucurbitacin D Induces G2/M Phase Arrest and Apoptosis via the ROS/p38 Pathway in Capan-1 Pancreatic Cancer Cell Line
title_full Cucurbitacin D Induces G2/M Phase Arrest and Apoptosis via the ROS/p38 Pathway in Capan-1 Pancreatic Cancer Cell Line
title_fullStr Cucurbitacin D Induces G2/M Phase Arrest and Apoptosis via the ROS/p38 Pathway in Capan-1 Pancreatic Cancer Cell Line
title_full_unstemmed Cucurbitacin D Induces G2/M Phase Arrest and Apoptosis via the ROS/p38 Pathway in Capan-1 Pancreatic Cancer Cell Line
title_short Cucurbitacin D Induces G2/M Phase Arrest and Apoptosis via the ROS/p38 Pathway in Capan-1 Pancreatic Cancer Cell Line
title_sort cucurbitacin d induces g2/m phase arrest and apoptosis via the ros/p38 pathway in capan-1 pancreatic cancer cell line
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7527894/
https://www.ncbi.nlm.nih.gov/pubmed/33029168
http://dx.doi.org/10.1155/2020/6571674
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