Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection

The CRISPR-Cas12a RNA-guided complexes have tremendous potential for nucleic acid detection but are limited to the picomolar detection limit without an amplification step. Here, we develop a platform with engineered crRNAs and optimized conditions that enabled us to detect various clinically relevan...

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Detalles Bibliográficos
Autores principales: Nguyen, Long T., Smith, Brianna M., Jain, Piyush K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7528031/
https://www.ncbi.nlm.nih.gov/pubmed/32999292
http://dx.doi.org/10.1038/s41467-020-18615-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7528031/
https://www.ncbi.nlm.nih.gov/pubmed/32999292
http://dx.doi.org/10.1038/s41467-020-18615-1

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