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Non-specific amplification of human DNA is a major challenge for 16S rRNA gene sequence analysis

The targeted sequencing of the 16S rRNA gene is one of the most frequently employed techniques in the field of microbial ecology, with the bacterial communities of a wide variety of niches in the human body have been characterised in this way. This is performed by targeting one or more hypervariable...

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Detalles Bibliográficos
Autores principales: Walker, Sidney P., Barrett, Maurice, Hogan, Glenn, Flores Bueso, Yensi, Claesson, Marcus J., Tangney, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7529756/
https://www.ncbi.nlm.nih.gov/pubmed/33004967
http://dx.doi.org/10.1038/s41598-020-73403-7