Characterization and mitigation of fragmentation enzyme-induced dual stranded artifacts

High-throughput short-read sequencing relies on fragmented DNA for optimal sampling of input nucleic acid. Several vendors now offer proprietary enzyme cocktails as a cheaper and more streamlined method of fragmentation when compared to acoustic shearing. We have discovered that these enzymes induce...

Descripción completa

Detalles Bibliográficos
Autores principales: Gregory, Thomas, Ngankeu, Apollinaire, Orwick, Shelley, Kautto, Esko A, Woyach, Jennifer A, Byrd, John C, Blachly, James S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Standard Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7531576/
https://www.ncbi.nlm.nih.gov/pubmed/33043294
http://dx.doi.org/10.1093/nargab/lqaa070

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7531576/
https://www.ncbi.nlm.nih.gov/pubmed/33043294
http://dx.doi.org/10.1093/nargab/lqaa070

Ejemplares similares