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Generation of a conditional mutant knock-in under the control of the natural promoter using CRISPR-Cas9 and Cre-Lox systems

Modulation of gene activity by creating mutations has contributed significantly to the understanding of protein functions. Oftentimes, however, mutational analyses use overexpression studies, in which proteins are taken out of their normal contexts and stoichiometries. In the present work, we sought...

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Detalles Bibliográficos
Autores principales: Thakur, Vijay S., Welford, Scott M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7531807/
https://www.ncbi.nlm.nih.gov/pubmed/33007045
http://dx.doi.org/10.1371/journal.pone.0240256