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A CRISPR/dCas9 toolkit for functional analysis of maize genes
BACKGROUND: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system has become a powerful tool for functional genomics in plants. The RNA-guided nuclease can be used to not only generate precise genomic mutations, but also to manipulate gene expression when present as a de...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7532566/ https://www.ncbi.nlm.nih.gov/pubmed/33024447 http://dx.doi.org/10.1186/s13007-020-00675-5 |