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Lost in translation: the quest for Nitrosomonas cluster 7‐specific amoA primers and TaqMan probes

The choice of primer and TaqMan probes to quantify ammonia‐oxidizing bacteria (AOB) in environmental samples is of crucial importance. The re‐evaluation of primer pairs based on current genomic sequences used for quantification of the amoA gene revealed (1) significant misrepresentations of the AOB...

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Detalles Bibliográficos
Autores principales: Orschler, Laura, Agrawal, Shelesh, Lackner, Susanne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533338/
https://www.ncbi.nlm.nih.gov/pubmed/32686322
http://dx.doi.org/10.1111/1751-7915.13627