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Rapid Self-Selecting and Clone-Free Integration of Transgenes into Engineered CRISPR Safe Harbor Locations in Caenorhabditis elegans

Precision genome editing for model organisms has revolutionized functional analysis and validation of a wide variety of molecular systems. To date, the capacity to insert single-copy transgenes into the model nematode Caenorhabditis elegans has focused on utilizing either transposable elements or CR...

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Detalles Bibliográficos
Autores principales: Stevenson, Zachary C., Moerdyk-Schauwecker, Megan J., Jamison, Brennen, Phillips, Patrick C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7534419/
https://www.ncbi.nlm.nih.gov/pubmed/32816924
http://dx.doi.org/10.1534/g3.120.401400