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Rapid Self-Selecting and Clone-Free Integration of Transgenes into Engineered CRISPR Safe Harbor Locations in Caenorhabditis elegans
Precision genome editing for model organisms has revolutionized functional analysis and validation of a wide variety of molecular systems. To date, the capacity to insert single-copy transgenes into the model nematode Caenorhabditis elegans has focused on utilizing either transposable elements or CR...
Autores principales: | Stevenson, Zachary C., Moerdyk-Schauwecker, Megan J., Jamison, Brennen, Phillips, Patrick C. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Genetics Society of America
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7534419/ https://www.ncbi.nlm.nih.gov/pubmed/32816924 http://dx.doi.org/10.1534/g3.120.401400 |
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