Unscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy

Determining nanoscale protein distribution via Photoactivated Localization Microscopy (PALM) mandates precise knowledge of the applied fluorophore’s blinking properties to counteract overcounting artifacts that distort the resulting biomolecular distributions. Here, we present a readily applicable m...

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Detalles Bibliográficos
Autores principales: Platzer, René, Rossboth, Benedikt K., Schneider, Magdalena C., Sevcsik, Eva, Baumgart, Florian, Stockinger, Hannes, Schütz, Gerhard J., Huppa, Johannes B., Brameshuber, Mario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7536177/
https://www.ncbi.nlm.nih.gov/pubmed/33020470
http://dx.doi.org/10.1038/s41467-020-18726-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7536177/
https://www.ncbi.nlm.nih.gov/pubmed/33020470
http://dx.doi.org/10.1038/s41467-020-18726-9

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