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Unscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy
Determining nanoscale protein distribution via Photoactivated Localization Microscopy (PALM) mandates precise knowledge of the applied fluorophore’s blinking properties to counteract overcounting artifacts that distort the resulting biomolecular distributions. Here, we present a readily applicable m...
Autores principales: | Platzer, René, Rossboth, Benedikt K., Schneider, Magdalena C., Sevcsik, Eva, Baumgart, Florian, Stockinger, Hannes, Schütz, Gerhard J., Huppa, Johannes B., Brameshuber, Mario |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7536177/ https://www.ncbi.nlm.nih.gov/pubmed/33020470 http://dx.doi.org/10.1038/s41467-020-18726-9 |
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