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Distinct signatures of gut microbiome and metabolites associated with significant fibrosis in non-obese NAFLD

Nonalcoholic fatty liver disease (NAFLD) is associated with obesity but also found in non-obese individuals. Gut microbiome profiles of 171 Asians with biopsy-proven NAFLD and 31 non-NAFLD controls are analyzed using 16S rRNA sequencing; an independent Western cohort is used for external validation....

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Autores principales: Lee, Giljae, You, Hyun Ju, Bajaj, Jasmohan S., Joo, Sae Kyung, Yu, Junsun, Park, Seoyeon, Kang, Hyena, Park, Jeong Hwan, Kim, Jung Ho, Lee, Dong Hyeon, Lee, Seonhwa, Kim, Won, Ko, GwangPyo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7536225/
https://www.ncbi.nlm.nih.gov/pubmed/33020474
http://dx.doi.org/10.1038/s41467-020-18754-5
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author Lee, Giljae
You, Hyun Ju
Bajaj, Jasmohan S.
Joo, Sae Kyung
Yu, Junsun
Park, Seoyeon
Kang, Hyena
Park, Jeong Hwan
Kim, Jung Ho
Lee, Dong Hyeon
Lee, Seonhwa
Kim, Won
Ko, GwangPyo
author_facet Lee, Giljae
You, Hyun Ju
Bajaj, Jasmohan S.
Joo, Sae Kyung
Yu, Junsun
Park, Seoyeon
Kang, Hyena
Park, Jeong Hwan
Kim, Jung Ho
Lee, Dong Hyeon
Lee, Seonhwa
Kim, Won
Ko, GwangPyo
author_sort Lee, Giljae
collection PubMed
description Nonalcoholic fatty liver disease (NAFLD) is associated with obesity but also found in non-obese individuals. Gut microbiome profiles of 171 Asians with biopsy-proven NAFLD and 31 non-NAFLD controls are analyzed using 16S rRNA sequencing; an independent Western cohort is used for external validation. Subjects are classified into three subgroups according to histological spectra of NAFLD or fibrosis severity. Significant alterations in microbiome diversity are observed according to fibrosis severity in non-obese, but not obese, subjects. Ruminococcaceae and Veillonellaceae are the main microbiota associated with fibrosis severity in non-obese subjects. Furthermore, stool bile acids and propionate are elevated, especially in non-obese subjects with significant fibrosis. Fibrosis-related Ruminococcaceae and Veillonellaceae species undergo metagenome sequencing, and four representative species are administered in three mouse NAFLD models to evaluate their effects on liver damage. This study provides the evidence for the role of the microbiome in the liver fibrosis pathogenesis, especially in non-obese subjects.
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spelling pubmed-75362252020-10-19 Distinct signatures of gut microbiome and metabolites associated with significant fibrosis in non-obese NAFLD Lee, Giljae You, Hyun Ju Bajaj, Jasmohan S. Joo, Sae Kyung Yu, Junsun Park, Seoyeon Kang, Hyena Park, Jeong Hwan Kim, Jung Ho Lee, Dong Hyeon Lee, Seonhwa Kim, Won Ko, GwangPyo Nat Commun Article Nonalcoholic fatty liver disease (NAFLD) is associated with obesity but also found in non-obese individuals. Gut microbiome profiles of 171 Asians with biopsy-proven NAFLD and 31 non-NAFLD controls are analyzed using 16S rRNA sequencing; an independent Western cohort is used for external validation. Subjects are classified into three subgroups according to histological spectra of NAFLD or fibrosis severity. Significant alterations in microbiome diversity are observed according to fibrosis severity in non-obese, but not obese, subjects. Ruminococcaceae and Veillonellaceae are the main microbiota associated with fibrosis severity in non-obese subjects. Furthermore, stool bile acids and propionate are elevated, especially in non-obese subjects with significant fibrosis. Fibrosis-related Ruminococcaceae and Veillonellaceae species undergo metagenome sequencing, and four representative species are administered in three mouse NAFLD models to evaluate their effects on liver damage. This study provides the evidence for the role of the microbiome in the liver fibrosis pathogenesis, especially in non-obese subjects. Nature Publishing Group UK 2020-10-05 /pmc/articles/PMC7536225/ /pubmed/33020474 http://dx.doi.org/10.1038/s41467-020-18754-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lee, Giljae
You, Hyun Ju
Bajaj, Jasmohan S.
Joo, Sae Kyung
Yu, Junsun
Park, Seoyeon
Kang, Hyena
Park, Jeong Hwan
Kim, Jung Ho
Lee, Dong Hyeon
Lee, Seonhwa
Kim, Won
Ko, GwangPyo
Distinct signatures of gut microbiome and metabolites associated with significant fibrosis in non-obese NAFLD
title Distinct signatures of gut microbiome and metabolites associated with significant fibrosis in non-obese NAFLD
title_full Distinct signatures of gut microbiome and metabolites associated with significant fibrosis in non-obese NAFLD
title_fullStr Distinct signatures of gut microbiome and metabolites associated with significant fibrosis in non-obese NAFLD
title_full_unstemmed Distinct signatures of gut microbiome and metabolites associated with significant fibrosis in non-obese NAFLD
title_short Distinct signatures of gut microbiome and metabolites associated with significant fibrosis in non-obese NAFLD
title_sort distinct signatures of gut microbiome and metabolites associated with significant fibrosis in non-obese nafld
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7536225/
https://www.ncbi.nlm.nih.gov/pubmed/33020474
http://dx.doi.org/10.1038/s41467-020-18754-5
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