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Single-cell analysis of germinal-center B cells informs on lymphoma cell of origin and outcome

In response to T cell–dependent antigens, mature B cells are stimulated to form germinal centers (GCs), the sites of B cell affinity maturation and the cell of origin (COO) of most B cell lymphomas. To explore the dynamics of GC B cell development beyond the known dark zone and light zone compartmen...

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Autores principales: Holmes, Antony B., Corinaldesi, Clarissa, Shen, Qiong, Kumar, Rahul, Compagno, Nicolo, Wang, Zhong, Nitzan, Mor, Grunstein, Eli, Pasqualucci, Laura, Dalla-Favera, Riccardo, Basso, Katia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7537389/
https://www.ncbi.nlm.nih.gov/pubmed/32603407
http://dx.doi.org/10.1084/jem.20200483
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author Holmes, Antony B.
Corinaldesi, Clarissa
Shen, Qiong
Kumar, Rahul
Compagno, Nicolo
Wang, Zhong
Nitzan, Mor
Grunstein, Eli
Pasqualucci, Laura
Dalla-Favera, Riccardo
Basso, Katia
author_facet Holmes, Antony B.
Corinaldesi, Clarissa
Shen, Qiong
Kumar, Rahul
Compagno, Nicolo
Wang, Zhong
Nitzan, Mor
Grunstein, Eli
Pasqualucci, Laura
Dalla-Favera, Riccardo
Basso, Katia
author_sort Holmes, Antony B.
collection PubMed
description In response to T cell–dependent antigens, mature B cells are stimulated to form germinal centers (GCs), the sites of B cell affinity maturation and the cell of origin (COO) of most B cell lymphomas. To explore the dynamics of GC B cell development beyond the known dark zone and light zone compartments, we performed single-cell (sc) transcriptomic analysis on human GC B cells and identified multiple functionally linked subpopulations, including the distinct precursors of memory B cells and plasma cells. The gene expression signatures associated with these GC subpopulations were effective in providing a sc-COO for ∼80% of diffuse large B cell lymphomas (DLBCLs) and identified novel prognostic subgroups of DLBCL.
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spelling pubmed-75373892021-04-05 Single-cell analysis of germinal-center B cells informs on lymphoma cell of origin and outcome Holmes, Antony B. Corinaldesi, Clarissa Shen, Qiong Kumar, Rahul Compagno, Nicolo Wang, Zhong Nitzan, Mor Grunstein, Eli Pasqualucci, Laura Dalla-Favera, Riccardo Basso, Katia J Exp Med Article In response to T cell–dependent antigens, mature B cells are stimulated to form germinal centers (GCs), the sites of B cell affinity maturation and the cell of origin (COO) of most B cell lymphomas. To explore the dynamics of GC B cell development beyond the known dark zone and light zone compartments, we performed single-cell (sc) transcriptomic analysis on human GC B cells and identified multiple functionally linked subpopulations, including the distinct precursors of memory B cells and plasma cells. The gene expression signatures associated with these GC subpopulations were effective in providing a sc-COO for ∼80% of diffuse large B cell lymphomas (DLBCLs) and identified novel prognostic subgroups of DLBCL. Rockefeller University Press 2020-06-30 /pmc/articles/PMC7537389/ /pubmed/32603407 http://dx.doi.org/10.1084/jem.20200483 Text en © 2020 Holmes et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Holmes, Antony B.
Corinaldesi, Clarissa
Shen, Qiong
Kumar, Rahul
Compagno, Nicolo
Wang, Zhong
Nitzan, Mor
Grunstein, Eli
Pasqualucci, Laura
Dalla-Favera, Riccardo
Basso, Katia
Single-cell analysis of germinal-center B cells informs on lymphoma cell of origin and outcome
title Single-cell analysis of germinal-center B cells informs on lymphoma cell of origin and outcome
title_full Single-cell analysis of germinal-center B cells informs on lymphoma cell of origin and outcome
title_fullStr Single-cell analysis of germinal-center B cells informs on lymphoma cell of origin and outcome
title_full_unstemmed Single-cell analysis of germinal-center B cells informs on lymphoma cell of origin and outcome
title_short Single-cell analysis of germinal-center B cells informs on lymphoma cell of origin and outcome
title_sort single-cell analysis of germinal-center b cells informs on lymphoma cell of origin and outcome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7537389/
https://www.ncbi.nlm.nih.gov/pubmed/32603407
http://dx.doi.org/10.1084/jem.20200483
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