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Highly efficient homology‐directed repair using CRISPR/Cpf1‐geminiviral replicon in tomato
Genome editing via the homology‐directed repair (HDR) pathway in somatic plant cells is very inefficient compared with error‐prone repair by nonhomologous end joining (NHEJ). Here, we increased HDR‐based genome editing efficiency approximately threefold compared with a Cas9‐based single‐replicon sys...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7540044/ https://www.ncbi.nlm.nih.gov/pubmed/32176419 http://dx.doi.org/10.1111/pbi.13373 |