Cargando…

TIR/BB-loop mimetic AS-1 protects vascular endothelial cells from injury induced by hypoxia/reoxygenation

Morphological and functional abnormalities of vascular endothelial cells (VECs) are risk factors of ischemia-reperfusion in skin flaps. Signaling pathway mediated by interleukin-1 receptor (IL-1R) is essential to hypoxia/reoxygenation (H/R) injury of VECs. While the TIR/BB-loop mimetic (AS-1) disrup...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Zhijia, Hou, Yuxing, Li, Jiantao, Tang, Chao, Que, Linli, Tan, Qian, Li, Yuehua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Editorial Department of Journal of Biomedical Research 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7540235/
https://www.ncbi.nlm.nih.gov/pubmed/32594023
http://dx.doi.org/10.7555/JBR.33.20190030
_version_ 1783591162468106240
author Zhang, Zhijia
Hou, Yuxing
Li, Jiantao
Tang, Chao
Que, Linli
Tan, Qian
Li, Yuehua
author_facet Zhang, Zhijia
Hou, Yuxing
Li, Jiantao
Tang, Chao
Que, Linli
Tan, Qian
Li, Yuehua
author_sort Zhang, Zhijia
collection PubMed
description Morphological and functional abnormalities of vascular endothelial cells (VECs) are risk factors of ischemia-reperfusion in skin flaps. Signaling pathway mediated by interleukin-1 receptor (IL-1R) is essential to hypoxia/reoxygenation (H/R) injury of VECs. While the TIR/BB-loop mimetic (AS-1) disrupts the interaction between IL-1R and myeloid differentiation primary-response protein 88 (MyD88), its role in the VECs dysfunction under H/R is unclear. In this study, we first showed that there was an infiltration of inflammatory cells and the apoptosis of VECs by using a skin flap section from patients who received flap transplantation. We then showed that the H/R treatment induced apoptosis and loss of cell migration of endothelial cell line H926 were attenuated by AS-1. Furthermore, our data suggested that AS-1 inhibits the interaction between IL-1R and MyD88, and subsequent phosphorylation of IκB and p38 pathway, as well as the nuclear localization of NF-KB subunit p65/p50. Thus, this study indicated that the protective role of AS-1 in H/R induced cellular injury may be due to the AS-1 mediated down-regulation of IL-1R signaling pathway.
format Online
Article
Text
id pubmed-7540235
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Editorial Department of Journal of Biomedical Research
record_format MEDLINE/PubMed
spelling pubmed-75402352020-10-30 TIR/BB-loop mimetic AS-1 protects vascular endothelial cells from injury induced by hypoxia/reoxygenation Zhang, Zhijia Hou, Yuxing Li, Jiantao Tang, Chao Que, Linli Tan, Qian Li, Yuehua J Biomed Res Original Article Morphological and functional abnormalities of vascular endothelial cells (VECs) are risk factors of ischemia-reperfusion in skin flaps. Signaling pathway mediated by interleukin-1 receptor (IL-1R) is essential to hypoxia/reoxygenation (H/R) injury of VECs. While the TIR/BB-loop mimetic (AS-1) disrupts the interaction between IL-1R and myeloid differentiation primary-response protein 88 (MyD88), its role in the VECs dysfunction under H/R is unclear. In this study, we first showed that there was an infiltration of inflammatory cells and the apoptosis of VECs by using a skin flap section from patients who received flap transplantation. We then showed that the H/R treatment induced apoptosis and loss of cell migration of endothelial cell line H926 were attenuated by AS-1. Furthermore, our data suggested that AS-1 inhibits the interaction between IL-1R and MyD88, and subsequent phosphorylation of IκB and p38 pathway, as well as the nuclear localization of NF-KB subunit p65/p50. Thus, this study indicated that the protective role of AS-1 in H/R induced cellular injury may be due to the AS-1 mediated down-regulation of IL-1R signaling pathway. Editorial Department of Journal of Biomedical Research 2020-09 /pmc/articles/PMC7540235/ /pubmed/32594023 http://dx.doi.org/10.7555/JBR.33.20190030 Text en Copyright and License information: Journal of Biomedical Research, CAS Springer-Verlag Berlin Heidelberg 2020 http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Original Article
Zhang, Zhijia
Hou, Yuxing
Li, Jiantao
Tang, Chao
Que, Linli
Tan, Qian
Li, Yuehua
TIR/BB-loop mimetic AS-1 protects vascular endothelial cells from injury induced by hypoxia/reoxygenation
title TIR/BB-loop mimetic AS-1 protects vascular endothelial cells from injury induced by hypoxia/reoxygenation
title_full TIR/BB-loop mimetic AS-1 protects vascular endothelial cells from injury induced by hypoxia/reoxygenation
title_fullStr TIR/BB-loop mimetic AS-1 protects vascular endothelial cells from injury induced by hypoxia/reoxygenation
title_full_unstemmed TIR/BB-loop mimetic AS-1 protects vascular endothelial cells from injury induced by hypoxia/reoxygenation
title_short TIR/BB-loop mimetic AS-1 protects vascular endothelial cells from injury induced by hypoxia/reoxygenation
title_sort tir/bb-loop mimetic as-1 protects vascular endothelial cells from injury induced by hypoxia/reoxygenation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7540235/
https://www.ncbi.nlm.nih.gov/pubmed/32594023
http://dx.doi.org/10.7555/JBR.33.20190030
work_keys_str_mv AT zhangzhijia tirbbloopmimeticas1protectsvascularendothelialcellsfrominjuryinducedbyhypoxiareoxygenation
AT houyuxing tirbbloopmimeticas1protectsvascularendothelialcellsfrominjuryinducedbyhypoxiareoxygenation
AT lijiantao tirbbloopmimeticas1protectsvascularendothelialcellsfrominjuryinducedbyhypoxiareoxygenation
AT tangchao tirbbloopmimeticas1protectsvascularendothelialcellsfrominjuryinducedbyhypoxiareoxygenation
AT quelinli tirbbloopmimeticas1protectsvascularendothelialcellsfrominjuryinducedbyhypoxiareoxygenation
AT tanqian tirbbloopmimeticas1protectsvascularendothelialcellsfrominjuryinducedbyhypoxiareoxygenation
AT liyuehua tirbbloopmimeticas1protectsvascularendothelialcellsfrominjuryinducedbyhypoxiareoxygenation