CRISPRoff enables spatio-temporal control of CRISPR editing

Following introduction of CRISPR-Cas9 components into a cell, genome editing occurs unabated until degradation of its component nucleic acids and proteins by cellular processes. This uncontrolled reaction can lead to unintended consequences including off-target editing and chromosomal translocations...

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Detalles Bibliográficos
Autores principales: Carlson-Stevermer, Jared, Kelso, Reed, Kadina, Anastasia, Joshi, Sahil, Rossi, Nicholas, Walker, John, Stoner, Rich, Maures, Travis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542431/
https://www.ncbi.nlm.nih.gov/pubmed/33028827
http://dx.doi.org/10.1038/s41467-020-18853-3
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author Carlson-Stevermer, Jared
Kelso, Reed
Kadina, Anastasia
Joshi, Sahil
Rossi, Nicholas
Walker, John
Stoner, Rich
Maures, Travis
author_facet Carlson-Stevermer, Jared
Kelso, Reed
Kadina, Anastasia
Joshi, Sahil
Rossi, Nicholas
Walker, John
Stoner, Rich
Maures, Travis
author_sort Carlson-Stevermer, Jared
collection PubMed
description Following introduction of CRISPR-Cas9 components into a cell, genome editing occurs unabated until degradation of its component nucleic acids and proteins by cellular processes. This uncontrolled reaction can lead to unintended consequences including off-target editing and chromosomal translocations. To address this, we develop a method for light-induced degradation of sgRNA termed CRISPRoff. Here we show that light-induced inactivation of ribonucleoprotein attenuates genome editing within cells and allows for titratable levels of editing efficiency and spatial patterning via selective illumination.
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spelling pubmed-75424312020-10-19 CRISPRoff enables spatio-temporal control of CRISPR editing Carlson-Stevermer, Jared Kelso, Reed Kadina, Anastasia Joshi, Sahil Rossi, Nicholas Walker, John Stoner, Rich Maures, Travis Nat Commun Article Following introduction of CRISPR-Cas9 components into a cell, genome editing occurs unabated until degradation of its component nucleic acids and proteins by cellular processes. This uncontrolled reaction can lead to unintended consequences including off-target editing and chromosomal translocations. To address this, we develop a method for light-induced degradation of sgRNA termed CRISPRoff. Here we show that light-induced inactivation of ribonucleoprotein attenuates genome editing within cells and allows for titratable levels of editing efficiency and spatial patterning via selective illumination. Nature Publishing Group UK 2020-10-07 /pmc/articles/PMC7542431/ /pubmed/33028827 http://dx.doi.org/10.1038/s41467-020-18853-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Carlson-Stevermer, Jared
Kelso, Reed
Kadina, Anastasia
Joshi, Sahil
Rossi, Nicholas
Walker, John
Stoner, Rich
Maures, Travis
CRISPRoff enables spatio-temporal control of CRISPR editing
title CRISPRoff enables spatio-temporal control of CRISPR editing
title_full CRISPRoff enables spatio-temporal control of CRISPR editing
title_fullStr CRISPRoff enables spatio-temporal control of CRISPR editing
title_full_unstemmed CRISPRoff enables spatio-temporal control of CRISPR editing
title_short CRISPRoff enables spatio-temporal control of CRISPR editing
title_sort crisproff enables spatio-temporal control of crispr editing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542431/
https://www.ncbi.nlm.nih.gov/pubmed/33028827
http://dx.doi.org/10.1038/s41467-020-18853-3
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