Creation of Golden Gate constructs for gene doctoring

BACKGROUND: Gene doctoring is an efficient recombination-based genetic engineering approach to mutagenesis of the bacterial chromosome that combines the λ-Red recombination system with a suicide donor plasmid that is cleaved in vivo to generate linear DNA fragments suitable for recombination. The us...

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Detalles Bibliográficos
Autores principales: Thomson, Nicholas M., Zhang, Chuanzhen, Trampari, Eleftheria, Pallen, Mark J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542709/
https://www.ncbi.nlm.nih.gov/pubmed/33028286
http://dx.doi.org/10.1186/s12896-020-00648-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542709/
https://www.ncbi.nlm.nih.gov/pubmed/33028286
http://dx.doi.org/10.1186/s12896-020-00648-5

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