Protocol to Design, Clone, and Validate sgRNAs for In Vivo Reverse Genetic Studies

AAV-CRISPR/Cas9 permits gene mutagenesis in the adult CNS. Current methods determining in vivo on-target mutagenesis have been limited by the ability to isolate virally transduced cells. This protocol optimizes a workflow for the design, cloning, and validation of sgRNAs delivered by AAVs in vivo th...

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Detalles Bibliográficos
Autores principales: Hunker, Avery C., Zweifel, Larry S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7544006/
https://www.ncbi.nlm.nih.gov/pubmed/33043306
http://dx.doi.org/10.1016/j.xpro.2020.100070

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7544006/
https://www.ncbi.nlm.nih.gov/pubmed/33043306
http://dx.doi.org/10.1016/j.xpro.2020.100070

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