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Protocol to Design, Clone, and Validate sgRNAs for In Vivo Reverse Genetic Studies
AAV-CRISPR/Cas9 permits gene mutagenesis in the adult CNS. Current methods determining in vivo on-target mutagenesis have been limited by the ability to isolate virally transduced cells. This protocol optimizes a workflow for the design, cloning, and validation of sgRNAs delivered by AAVs in vivo th...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7544006/ https://www.ncbi.nlm.nih.gov/pubmed/33043306 http://dx.doi.org/10.1016/j.xpro.2020.100070 |