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Rapid, robust plasmid verification by de novo assembly of short sequencing reads

Plasmids are a foundational tool for basic and applied research across all subfields of biology. Increasingly, researchers in synthetic biology are relying on and developing massive libraries of plasmids as vectors for directed evolution, combinatorial gene circuit tests, and for CRISPR multiplexing...

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Detalles Bibliográficos
Autores principales: Gallegos, Jenna E, Rogers, Mark F, Cialek, Charlotte A, Peccoud, Jean
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7544192/
https://www.ncbi.nlm.nih.gov/pubmed/32890398
http://dx.doi.org/10.1093/nar/gkaa727