Unbiased investigation of specificities of prime editing systems in human cells

Prime editors (PEs) enable targeted precise editing, including the generation of substitutions, insertions and deletions, in eukaryotic genomes. However, their genome-wide specificity has not been explored. Here, we developed Nickase-based Digenome-seq (nDigenome-seq), an in vitro assay that uses wh...

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Detalles Bibliográficos
Autores principales: Kim, Do Yon, Moon, Su Bin, Ko, Jeong-Heon, Kim, Yong-Sam, Kim, Daesik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7544197/
https://www.ncbi.nlm.nih.gov/pubmed/32941652
http://dx.doi.org/10.1093/nar/gkaa764