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Comparison of senescence progression in mesenchymal cells from human umbilical cord walls measured by immunofluorescence and flow cytometry of p16 and p21

OBJECTIVE: To follow the expansion of mesenchymal stem cells from umbilical cords by two classic senescence markers, p16 (INK4A) and p21 (CDKN1A), using practical, fast, and less expensive methods than the gold standard Western blotting technique, to evaluate its applicability in the laboratory. MET...

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Detalles Bibliográficos
Autores principales: da Silva, Aline, Piccinato, Carla de Azevedo, Sardinha, Luiz Roberto, Aloia, Thiago Pinheiro Arrais, Goldberg, Anna Carla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Israelita de Ensino e Pesquisa Albert Einstein 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7546682/
https://www.ncbi.nlm.nih.gov/pubmed/33084793
http://dx.doi.org/10.31744/einstein_journal/2020AO5236