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A specific combination of dual index adaptors decreases the sensitivity of amplicon sequencing with the Illumina platform

Amplicon sequencing is a powerful approach in microbiome studies as it detects live organisms with high sensitivity. This approach determines the composition of sequence variants of marker genes using high-throughput DNA sequencers. The use of dual index adaptors is the fundamental technique for poo...

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Detalles Bibliográficos
Autores principales: Hirose, Yuu, Shiozaki, Takuhei, Hamano, Itsuki, Yoshihara, Shizue, Tokumoto, Hayato, Eki, Toshihiko, Harada, Naomi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547650/
https://www.ncbi.nlm.nih.gov/pubmed/32810209
http://dx.doi.org/10.1093/dnares/dsaa017