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A specific combination of dual index adaptors decreases the sensitivity of amplicon sequencing with the Illumina platform
Amplicon sequencing is a powerful approach in microbiome studies as it detects live organisms with high sensitivity. This approach determines the composition of sequence variants of marker genes using high-throughput DNA sequencers. The use of dual index adaptors is the fundamental technique for poo...
Autores principales: | Hirose, Yuu, Shiozaki, Takuhei, Hamano, Itsuki, Yoshihara, Shizue, Tokumoto, Hayato, Eki, Toshihiko, Harada, Naomi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547650/ https://www.ncbi.nlm.nih.gov/pubmed/32810209 http://dx.doi.org/10.1093/dnares/dsaa017 |
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